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Cell culture Human hepatoma HepG2 and SMMC-7721 cell lines, purchased from American Type Culture Collection (Manassas, VA), were grown in RPMI 1640 supplemented with 10% FBS, 100 U/mL benzyl penicillin and 100 U/mL of streptomycin, pH 7.4 in a humidified atmosphere with 5% CO 2 at 37C

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Subsequent in vivo and in vitro experiments confirmed that the transmembrane protein 164 ( TMEM164 ) gene might be mainly involved in the positive regulation of IMF deposition and have a certain negative regulatory effect on AF deposition

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